Development and characterization of microsatellite loci for the Neotropical orchid Trichocentrum pumilum
نویسندگان
چکیده
منابع مشابه
Cross-amplification and characterization of microsatellite loci for the Neotropical orchid genus Epidendrum
In this study we tested the cross-amplification of 33 microsatellite loci previously developed for two closely related Neotropical orchid genera (Epidendrum and Laelia). A set of ten loci were polymorphic across five examined species (20 individuals each) with 2 to 15 alleles per locus. The mean expected and observed heterozygosity (average across species) ranged from 0.34 to 0.82 and from 0.27...
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PREMISE OF THE STUDY Twenty-six microsatellite markers were developed for the endangered orchid Cypripedium japonicum (Orchidaceae) to estimate the clonal diversity and genetic structure of the remaining populations in Japan. METHODS AND RESULTS Microsatellite loci of C. japonicum were isolated using Ion Personal Genome Machine (PGM) sequencing. The primer sets were tested on 55 ramets sample...
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We report eight (CA) 10 − 35 unlinked microsatellite loci from the Neotropical freshwater catfish, Pimelodella chagresi (Siluriformes: Pimelodidae). These loci were characterized with 23 individuals collected in Panama. Number of alleles per locus varied from 7 to 23 (mean = 12.9) and observed heterozygosity ranged from 0.522 to 0.909 (mean = 0.732). These loci will be used to investigate the e...
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Eight novel microsatellite loci from the genome of Fenneropenaeus penicillatus Alcock were developed using the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Thirty (30) wild individuals were used to analyze the polymorphism of these eight microsatellite markers. The results show that the number of alleles per locus and the polymor...
متن کاملDevelopment and characterization of microsatellite loci for Ficus hirta (Moraceae).
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ژورنال
عنوان ژورنال: Crop Breeding and Applied Biotechnology
سال: 2016
ISSN: 1984-7033
DOI: 10.1590/1984-70332016v16n4n52